anti itga7 alexa fluor 700  (R&D Systems)

Journal: Cell Reports Methods

Article Title: African pygmy mouse iPSCs as a model for in vitro embryogenesis, interspecies chimerism, and blastocyst complementation

doi: 10.1016/j.crmeth.2025.101293

Figure Lengend Snippet: Ablation of mouse PAX7 + cells promotes APM satellite cell formation in mouse-APM chimeras (A) Schematic of experimental design to preferentially obtain APM satellite cells in mouse-APM chimeras through elimination of host mouse PAX7 + cells during postnatal growth. (B) Representative photos of mouse-APM chimeras generated from Pax7 Cre/ERT2 ; Rosa26 LSL-DTA blastocysts. Scale bars, 1 cm. (C) Representative bright-field and fluorescence overlay images of tibialis anterior (TA) muscles from the indicated animals. Scale bars, 5 mm. (D) Immunostaining images of TA muscle cross-sections for the indicated markers in the specified chimeras. Note the presence of PAX7 + /mCHERRY + cells with and without tamoxifen treatment. Scale bars, 100 μm. (E) Representative FACS plots displaying the percentage of mCHERRY + satellite cells within the ITGA7 + satellite cell population of a tamoxifen-treated vs. a B6 chimera. (F) Graph showing quantification of the representative FACS plots shown in (E) for a larger group of chimeras. Data are shown as mean ± SD. N = 3 tamoxifen-treated chimeras and N = 8 B6 chimeras. ∗∗ p ≤ 0.01. (G) Uniform manifold approximation and projection (UMAP) based on scRNA-seq of all cell populations in tamoxifen-treated mouse-APM chimera skeletal muscles, colored by the indicated cell type. SCs, satellite cells; FAPs, fibro-adipogenic progenitors. (H) Dot plot showing individual gene expression in mouse-APM cell populations used to annotate the UMAP shown in (G). (I) UMAP of all cells from skeletal muscles of a mouse-APM chimera, colored by detection of the H2B-mCherry transcript. (J) Representative bright-field and fluorescence overlay images of FACS-purified ITGA7 + /mCHERRY + and ITGA7 + /mCHERRY − myoblasts. Scale bars, 100 μm. (K) Representative immunostaining images for the skeletal muscle differentiation marker ACTN2 in ITGA7 + /mCHERRY + myoblast-derived myotubes. Scale bars, 100 μm.

Article Snippet: For satellite cell FACS-purification, cells were stained with conjugated anti-mouse LY-6A/E (SCA1) (1:100, Thermo Fisher, 11-5981-82), anti-mouse ITGA7 (1:100, R&D Systems, FAB3518S), anti-mouse CD45 (1:100, Biogend, 103121), anti-mouse PECAM1/CD31 (1:100, BioLegend, 102414), and DAPI (1:1000, Sigma-Aldrich MBD0015-1ML).

Techniques: Generated, Fluorescence, Muscles, Immunostaining, Gene Expression, Purification, Marker, Derivative Assay

Journal: In Vitro Cellular & Developmental Biology. Animal

Article Title: Correlation of molecular and cellular signatures in primary skeletal muscle satellite cells derived from lean and diet-induced obese mice

doi: 10.1007/s11626-025-01130-2

Figure Lengend Snippet: Ex vivo FACS-sorted satellite cells ( A , B ) and in vitro cultivation of satellite cell–derived myoblasts of CTL and DIO ( C ) with calculated doubling time ( D ). ( A ) FACS of propidium iodide–positive ( left ) and Itga7-positive ( right ) satellite cells of CTL-derived muscle tissue. ( B ) FACS of propidium iodide–positive ( left ) and Itga7-positive ( right ) satellite cells of DIO-derived muscle tissue. ( C ) Brightfield images of proliferating myoblasts on days 3 and 6 post plating of CTL ( upper line ) and DIO ( lower line ). Magnified single cells in upper right rectangles . Scale bar = 500 µm. ( D ) Analysis of doubling time of cultures of myoblasts of CTL and DIO over a period of 100 h.

Article Snippet: Integrin α7-PE-Vio770 (Itga7) , Miltenyi Biotec, Bergisch Gladbach, Germany; 130-120−812 , Mouse, monoclonal , MACS, FACS.

Techniques: Ex Vivo, In Vitro, Derivative Assay

Journal: In Vitro Cellular & Developmental Biology. Animal

Article Title: Correlation of molecular and cellular signatures in primary skeletal muscle satellite cells derived from lean and diet-induced obese mice

doi: 10.1007/s11626-025-01130-2

Figure Lengend Snippet: Myotube formation of satellite cell–derived myoblasts of CTL and DIO ( A ) with Western blot analysis of myogenic markers ( B – E ). ( A ) Brightfield images of differentiating myoblasts of CTL ( upper line ) and DIO ( lower line ) after 3 and 6 d of serum withdrawal. Magnified myotubes in upper right rectangles. Scale bar = 500 µm. ( B ) Western blot analysis of Myf5 protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. ( C ) Western blot analysis of MyoD protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. ( D ) Western blot analysis of myogenin protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. ( E ) Western blot analysis of Mf20 protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. ( F ) Western blot analysis of Itga7 protein in CTL and DIO satellite cell–derived myoblasts at 3 and 6 d of proliferation or differentiation, respectively. ( G ) Western blot analysis of CD56 protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. ( H ) Western blot analysis of Pax7 protein in CTL and DIO at 3 and 6 d of proliferation or differentiation, respectively. Fold change of normalized samples described as a quotient of each sample with standard control (proliferation 3 d).

Article Snippet: Integrin α7-PE-Vio770 (Itga7) , Miltenyi Biotec, Bergisch Gladbach, Germany; 130-120−812 , Mouse, monoclonal , MACS, FACS.

Techniques: Derivative Assay, Western Blot, Control